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7 M Sucrose 4 minutes 1M Sucrose Method 1 1ST COOLING STEP: Exposure to LN2 vapours (1 minute) 2ND COOLING STEP: Exposure to LN2 LN2 vapour LN2 Sequential cooling in liquid nitrogen (LN2) vapours followed by immersion into LN2 Method 2 1ST STEP: Packaging of sample into sterile sealed bags 2ND STEP: Exposure to LN2 Warming in 37°C 1 M sucrose solution (1 minute) COOLING-WARMING CYCLE LN2 One step cooling by direct immersion into LN2 0.
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